Delta sleep inducing peptide (DSIP)
For the synthesis of DSIP (Click to buy DSIP peptide for sale), the solution method and the solid phase method have been more mature. Although the enzymatic method has shortcomings, it has certain potential, after all, the genetic engineering method is still in the initial period. Both the solution method and the solid phase method have advantages and disadvantages. The advantages of the solution method are that the intermediate products of each step are purified. The purity of the products is better, the synthetic route is flexible and the synthetic quantity is large, and the disadvantages are too many steps and time consuming. The advantage of the solid phase method is that the intermediate product does not need separation and purification, saves time and effort, and is easy to automate. However, due to the presence of by-products such as decapitated peptides and peptides, the separation and purification of the final products are more difficult, and the cost is higher due to the overdosage of the base components. In addition, the amount of synthesis is limited. Because advantages and disadvantages of the two are complementary, the exploration of new solution methods, such as "residence solution method", has been explored and has obtained good results. Enzymatic method still can not replace chemical method at present, and it remains to be further developed. The advantage of enzymatic method is that carboxyl of carboxyl groups do not need activation and racemization is small. The side chains of amino acids of raw materials can sometimes not be protected. However, as the enzyme used is proteolytic enzyme, the possibility of enzymatic hydrolysis is increasing as the peptide chain grows. In recent years, the enzymatic peptide synthesis in non water medium has been a very useful exploration in trying to solve this problem and showed good prospects for application. The advantages of this method are that the enzyme can maintain its bioactivity in the organic solvent, the organic substrate has a larger solubility in the organic solvent, the thermal stability and storage stability in the organic solvent are significantly higher than that in the water, and the thermodynamic equilibrium of some reactions is moved to the desired direction, and the side reaction of water participation is inhibited. It is conducive to the separation and purification of products, inhibition of microbial pollution. Enzyme is insoluble in organic solvents, so it is conducive to the recovery and reuse of enzymes.